To detect the polycyclic aromatic hydrocarbons(PAHs) in soils accurately, the typical PAH pollutants of anthracene in soils was used as research targets, and the effect of soil particle size on the fluorescence characteristics was researched. Then, a correction method was established to reduce the influence of soil particle size on the standard curve of PAH. The fluorescence characteristics of anthracene in soils were studied and three fluorescence peaks were shown at 421 nm, 442 nm and 470 nm. Seven soil samples with different particle sizes contaminated with anthracene were prepared. On the basis of the perturbation of soil particle sizes, the synchronous and asynchronous two-dimensional(2D) correlation fluorescence spectra were calculated and the variations of the fluorescence intensity of anthracene and Rayleigh scattering intensity at 304 nm with soil particle sizes were studied. It was found that the fluorescence intensity of anthracene and Rayleigh scattering intensity enhance with the increase of soil particle sizes. Finally, the standard curves were established to determine the concentration of anthracene in 80 mesh and 160 mesh soils, respectively, and the fluorescence of anthracene was corrected by the Rayleigh scattering at 304 nm. The results show that the proposed corrected method effectively reduces the effect of soil particle size on the standard curve of anthracene.