Biometrics is becoming more and more important in the diagnosis and treatment of ophthalmic diseases. Monitoring the axial length of children and adolescents can help prevent and treat myopia, and the ophthalmic diseases that it causes. The accurate measurement of eye axial parameters is an important factor in visual quality that affects postoperative patients with eye diseases. In order to measure the parameters of the eye axis, the eye axis measurement method based on the time domainlow-coherence technique of time domain is presented. The prototype for high-precision and non-contact measurement is developed and the corresponding experiments are conducted. Using a combination of low-coherence interference technique and long coherent interference ranging technology, a fiber-optic low-coherence tomography measurement system is built using time-domain mechanical scanning. The repeatability error of standard lens thickness and mirror spacing measurements is less than 0.3 μm. In the experimental measurement of fish-eye axis parameters, the structure of the cornea, lens, and retina of the fish eye was clearly tested. The stability and repeatability of the system meets the design requirements. The tomographic measurement experiment on fish eyes successfully verified that the system can be used for the measurement of animal eye axis parameters.