For low material utilization and low optical efficiency of the quantum dots thin film transistor liquid crystal display (TFT-LCD) backlight, a new model is proposed to form quantum dot micro structure in light guide plate. The new structure make the blue light emitting diode (LED) pump into high color rendering index (CRI) white light. The backlight system is built, and the study on ray tracing simulation and parameter optimization shows that the new quantum dots backlight has the advantages of high optical efficiency and low cost. The experimental and theoretical results match well with each other.