Infrared and Laser Engineering, Volume. 48, Issue 11, 1114003(2019)
Design of variable light-sheet illumination system
To solve the problem of the single thickness of the light-sheet fluorescence microscopy, the design of the variable light-sheet illumination system was carried out based on zoom beam expanding. First, each group of the light-sheet illumination system was calculated using Gaussian optics to obtain the relation between the thickness of the light-sheet and the expansion ratio as well as the relation among the expansion ratio, the lateral magnification and focal length of each group. Then a variable light-sheet illumination system based on 10× beam expanding was designed to generate the light-sheet with continuously variable thickness and length. Finally, the parameters and uniformity of the light-sheet and the system tolerance were analyzed. The results show that the thickness of the continuously variable light-sheet is 3.33 μm to 33.3 μm, and the illumination uniformity is 0.65, 0.4 and 0.61 on the YOZ plane within 60% light-sheet height at low(1×), medium(6×) and high(10×) expansion ratios, respectively. Tolerance analysis shows that the maximum change of the light-sheet thickness is less than the 15% of design value at expansion ratio of 1×, less than 6% at 6× and 10×. The design realizes the continuous variation of the light sheet thickness, and the region of 60% light-sheet height is favorable for the observation of the sample.
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Jiang Lian, Rui Congshan, Zeng Chunmei, Ma Suodong. Design of variable light-sheet illumination system[J]. Infrared and Laser Engineering, 2019, 48(11): 1114003
Category: 光学设计
Received: Sep. 5, 2019
Accepted: Oct. 15, 2019
Published Online: Dec. 9, 2019
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