Spectroscopy and Spectral Analysis, Volume. 35, Issue 5, 1243(2015)

Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy

WANG Wei-ping1,2、*, CHAI A-li2, SHI Yan-xia2, XIE Xue-wen2, and LI Bao-ju2
Author Affiliations
  • 1[in Chinese]
  • 2[in Chinese]
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    Clubroot,caused by Plasmodiophora brassicae,is considered the most devastating soilborne disease in Brassica crops.It has emerged as a serious disease threatening the cruciferous crop production industry in China.Nowadays,the detection techniques for P.brassicae are laborious,time-consuming and low sensitivity.Rapid and effective detection methods are needed.The objective of this study is to develop a Fourier transform infrared spectrometer(FTIR) technique for detection of P.brassicae effectively and accurately.FTIR and Real-time PCR techniques were applied in quantitative detection of P.brassicae.Chinese cabbages were inoculated with P.brassicae.By analyzing the FTIR spectra of P.brassicae,infected clubroots and healthy roots,three specific bands 1 105,1 145 and 1 228 cm-1 were selected.According to the correlation between the peak areas at these sensitive bands and Real-time PCR Ct value,quantitative evaluation model of P.brassicae was established based on FTIR y=34.17+12.24x1-9.81x2-6.05x3,r=0.98(p<0.05).To validate accuracy of the model,10 clubroot samples were selected randomly from field,and detected by FTIR spectrum model,the results showed that the average error is 1.60%.This demonstrated that the FTIR technology is an available one for the quantitative detection of P.brassicae in clubroot,and it provides a new method for quantitative and quickly detection of Chinese cabbage clubroot.

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    WANG Wei-ping, CHAI A-li, SHI Yan-xia, XIE Xue-wen, LI Bao-ju. Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2015, 35(5): 1243

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    Paper Information

    Received: Oct. 30, 2014

    Accepted: --

    Published Online: May. 26, 2015

    The Author Email: Wei-ping WANG (wangweiping316@163.com)

    DOI:10.3964/j.issn.1000-0593(2015)05-1243-05

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