It is important to determinate uranium concentration in nuclear fuel reprocessing. The L-edge transmission spectrum of uranium, which is obtained by exposing uranium solution to an X-ray beam, can be applied for uranium determination. The L-edge transmission spectrum measurement system is introduced and the working curve for uranium determination is established with 1~200 g·L-1 uranium standard solutions. In order to verify the system, the precision, accuracy and stability experiments are carried out. The results show that the linear fitting parameter of the working curve is R2=0.999 9. The precision for 57.004 g·L-1 uranium solution is 0.21%, while the relative error is 0.32% for 38.255 g·L-1 uranium solution. It shows that 99.67% of the measurement results fall within μ±3 s range for 2.236 g·L-1.