Hydroxyl radical molecular beam was generated by DC pulsed high voltage discharge. The rotational resolution excitation fluorescence spectra of the H A 2Σ+-X 2Π (1, 0) band were observed by using second harmonious output of a nanosecond dye laser (around 282 nm). The rotational temperature of the (1, 0) band was determined to be (30±1) K based on the analysis of the intensity distribution of the H spectra. Moreover, the fluorescence decay curves of the (1, 1) band and the (0, 0) band and were obtained and the fluorescence lifetimes of A 2Σ+ (ｖ′=1) state and the X 2Π (ｖ″=0) state were fitted to be (637±16) and (675±13) ns, respectively.