Journal of Innovative Optical Health Sciences, Volume. 2, Issue 2, 189(2009)
DETECTION OF Ca2+-DEPENDENT NEURONAL ACTIVITY SIMULTANEOUSLY WITH DYNAMIC CHANGES IN CEREBRAL BLOOD VOLUME AND TISSUE OXYGENATION FROM THE LIVE RAT BRAIN
We present a catheter-based optical diffusion and fluorescence (ODF) probe to study the functional changes of the brain in vivo. This ODF probe enables the simultaneous detection of the multi-wavelength absorbance and fluorescence emission from the living rat brain. Our previous studies, including a transient stroke experiment of the rat brain as well as the brain response to cocaine, have established the feasibility of simultaneously determining changes in cerebral blood volume (CBV), tissue oxygenation (StO2) and intracellular calcium ([Ca2+]i, using the fluorescence indicator Rhod2). Here, we present our preliminary results of somatosensory response to electrical forepaw stimulation obtained from the rat cortical brain by using the ODF probe, which indicate that the probe could track brain activation by directly detecting [Ca2+]i along with separately distinguishing CBV and StO2 in real time. The changes of CBV, StO2 and [Ca2+]i are comparable with the blood-oxygen-level-dependent (BOLD) response to the stimulation obtained using functional magnetic resonance imaging (fMRI). However, the high temporal resolution of the optical methodology is advanced, thus providing a new modality for brain functional studies to understand the hemodynamic changes that underlie the neuronal activity.
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CONGWU DU, ZHONGCHI LUO, MEI YU, HELENE BENVENISTE, MELISSA TULLY, RUBING PAN, BRITTON CHANCE. DETECTION OF Ca2+-DEPENDENT NEURONAL ACTIVITY SIMULTANEOUSLY WITH DYNAMIC CHANGES IN CEREBRAL BLOOD VOLUME AND TISSUE OXYGENATION FROM THE LIVE RAT BRAIN[J]. Journal of Innovative Optical Health Sciences, 2009, 2(2): 189