In the present paper, low-energy N+ ions produced by low-energy ionic radiometer were used to simulate low-energy ions in astrospace implanting HeLa cell. Then, the effect and mechanism of low-energy ion on the cell were studied with FTIR spectroscopic analysis. In this study, low-energy ions were produced and accelerated in vacuum, and cells would be affected by vacuum when they were implanted by low-energy ions, so mineral oil was used to protect cells from water evaporation. Cells were collected after being implanted, then the change in the content and constructional form in cellular macromolecule with infrared spectrometry. Result indicated that the spectroscopic peak position of differently worked cells was obviously different as compared to the control cell (3 300 cm-1). Spectroscopic peak position of all samples except implanting 5×1014 N+·cm-2 removed to longer wavelength, and the peak position in vacuum of 2×1015 N+·cm-2 sample moved to 3 420 cm-1. In addition, the 1 378 and 2　360　cm-1 spectroscopic peak positions in control cell all moved to longer wavelength in every worked group. In a word, FTIR spectroscopic analysis indicates that low-energy ion implantation could arouse change in nucleic acid or protein in HeLa cell.