High dynamic range imaging technology is a research hotspot in the field of optical imaging. Digital microscopy device (DMD) has the characteristics of modulation incident ray spatial information. A high dynamic range imaging system with high-resolution is developed based on DMD, which could improve the dynamic range of the imaging system. At the same time, in order to reasonably and effectively control the DMD, the response curve of DMD modulation and CMOS image detector are calibrated by using mathematical analysis method, and the response curve calibration method of DMD camera is proposed according to the structure and imaging characteristics of the imaging system. The experimental results show that the imaging system has a dynamic range larger than the ordinary ones, which can reach more than 96 dB. The fast response function calibration of the DMD camera is also completed, which provides the powerful foundation for the adaptive adjustment of the DMD mask.