Laser & Optoelectronics Progress, Volume. 59, Issue 6, 0617009(2022)

Recent Advances in Structured Illumination Microscope Super-Resolution Image Reconstruction

Yujun Tang1,2, Linbo Wang2, Gang Wen2, and Hui Li1,2、*
Author Affiliations
  • 1School of Biomedical Engineering, University of Science and Technology of China, Suzhou , Jiangsu 215163, China
  • 2Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou , Jiangsu 215163, China
  • show less

    Structured illumination microscopy (SIM) has become one of the most popular super-resolution (SR) instruments for dynamic imaging of live cells. However, the final SR images of SIM depends heavily on the image reconstruction algorithms, which could dramatically affect the image quality. In the past five years, nearly 10 open-source software packages for SIM reconstruction have been developed with advantage on different situations. And deep learning based SIM reconstruction algorithms has also been reported. Understanding the principles and differences of each algorithm becomes a priority to select the appropriate algorithms for practical applications. This review firstly introduces the principle of SIM, and then presents the latest advances for the reference of SIM researchers and users from three aspects: estimation of structured illumination parameter, spectrum optimization, and deep learning based reconstruction. Finally, the remaining issues that need to be addressed further for high-quality SIM super-resolution image reconstruction are summarized.

    Tools

    Get Citation

    Copy Citation Text

    Yujun Tang, Linbo Wang, Gang Wen, Hui Li. Recent Advances in Structured Illumination Microscope Super-Resolution Image Reconstruction[J]. Laser & Optoelectronics Progress, 2022, 59(6): 0617009

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category: Medical Optics and Biotechnology

    Received: Jan. 4, 2022

    Accepted: Feb. 10, 2022

    Published Online: Mar. 8, 2022

    The Author Email: Li Hui (hui.li@sibet.ac.cn)

    DOI:10.3788/LOP202259.0617009

    Topics