Current research on photobiosafety focuses on artificial lighting with a constant spectral distribution. The influence of human transmittance with age is less considered. In the next 20 years, AMOLED will gradually become the mainstream display of smart-phones. This paper provides a theoretical reference for the personalized design and use of AMOLED from the perspective of blue light harm and rhythm effect. According to the function expression of the change of human eye transmittance with age given by the International Commission on Illumination (CIE) in 2012, the transmittance of the human eye was obtained for 11 different ages (1~100 years, with intervals of 10 years). We collected the original spectral distribution data of AMOLED at six different color temperatures (2 300, 2 700, 3 400, 4 100, 5 000, 6 500 K). We normalized the original spectral distribution data of AMOLED at different color temperatures. Then, we multiplied the normalized spectral distribution data of AMOLED at different color temperatures by the transmittance of human eyes at different ages and finally obtained the effective spectral distribution of AMOLED at different color temperatures on the retina of human eyes at different ages. We used the effective spectral distribution of the human retina to replace the original spectral distribution of the blue light hazard factor and rhythm factor in the calculation formula, and calculated the effective spectral distribution of the blue light hazard factor and rhythm factor of AMOLED in the human retina at different ages. Through the high-quality function fitting analysis, the blue light harm and rhythm effect of AMOLED with age change were studied. The research and analysis results show that: The blue light harm and rhythm effect of AMOLED decrease with the increase in the users age. For AMOLED with a color temperature of 6 500 K, the users age increases from 1 year to 100 years, and the blue light hazard factor and rhythm factor of the retinal effective spectrum decrease to 0.290 7 and 0.403 8 times respectively. When the age is more than 40 years old, the decrease of blue color hazard factors and rhythm factors of various temperatures with the increase of age is significantly accelerated. Under 6 different color temperatures, the average speed of blue light harm factor and rhythm factor decreased with age increase in 40~100 years old, which was 2.748 2 and 2.993 3 times as much as that in 1~40 years old. When the users age increases from 1 year to 100 years old, the average blue light hazard factor and the average rhythm factor of the 6 color temperatures decrease to 0.305 6 and 0.452 0 times respectively. Based on the above results: AMOLED greatly harms blue light and rhythm effect on young people, especially young users under 40 years old, so they should reduce their use time and pay attention to the harm and rhythm effect of blue light. The harm of blue light is more affected by age than rhythm effect. These conclusions may provide some theoretical references for relevant research.