A concentric glass spherical shell with a uniform thickness is usually chosen as the transparent window mounted in front of the optical lens of an airborne opto-electronic platform,becaused the misalignment of the spheric centers on inside and outside surfaces produced by the fabrication can cause additional pointing errors. In this paper, the theoretical expression of addition pointing error based on an optical path is derived and the effect of the misalignment of the spheric centers on inside and outside surfaces on the pointing accuracy of a sight axis is analyzed. Several kinds of experiments are undertaken and the data processing result of the experiment indicates that the measurement results are in agreement well with the theoretical results. When the concentric misalignment of a spherical center is 0.26 mm, the axial pointing error at a 45° field of view reaches 0.075°,which is higher than the error limitation 0.029° (0.5 mrad).Analyzed results also show that the concentric misalignment should be controlled below to 0.10 mm in order to reduce the pointing error to 0.017°(0.3 mrad). Based on the analyzed results,the inside and outside surfaces of the spherical shell is reprocessed,the experimental results demonstrate that the pointing error is acceptable when the concentric misalignment is controlled in 0.10 mm.