This paper analyses the information configuration in remote sensing observation data of imaging spectrometer,including the spectral character of ground surface,instrument parameter and atmosphere transmission and also discusses on the physical process and measurement chain of radiometric calibration of imaging spectrometer.Then according to the international updated method of ISO1993(E)' Express Guide of Measurement Uncertainty,the measurement uncertainty and combined standard uncertainty of eleven influence quantities of radiometric calibration are analyzed by the special equipment such as radiation standard,light source of integral sphere,spectroradiometer and in-flight calibration sets and so on.To accomplish the radiometric calibration,the measurement process of multi-measurement chain is set up.Experimental results show that the radiometric calibration of the imaging spectrometer requires special advanced equipment and technique.To reach the absolute accuracy of 5%～8%,the uncertainty of standard spectral irradiance must be 3%～5% and the measurement uncertainty of the other influence quantity items must be limited within 1%～2%.