Journal of Innovative Optical Health Sciences, Volume. 17, Issue 1, 2350030(2024)
In vivo measurement of NADH fluorescence lifetime in skeletal muscle via fiber-coupled time-correlated single photon counting
Nicotinamide adenine dinucleotide (NADH) is a cofactor that serves to shuttle electrons during metabolic processes such as glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation (OXPHOS). NADH is autofluorescent, and its fluorescence lifetime can be used to infer metabolic dynamics in living cells. Fiber-coupled time-correlated single photon counting (TCSPC) equipped with an implantable needle probe can be used to measure NADH lifetime in vivo, enabling investigation of changing metabolic demand during muscle contraction or tissue regeneration. This study illustrates a proof of concept for point-based, minimally-invasive NADH fluorescence lifetime measurement in vivo. Volumetric muscle loss (VML) injuries were created in the left tibialis anterior (TA) muscle of male Sprague Dawley rats. NADH lifetime measurements were collected before, during, and after a 30
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Kathryn M. Priest, Jacob V. Schluns, Nathania Nischal, Colton L. Gattis, Jeffrey C. Wolchok, Timothy J. Muldoon. In vivo measurement of NADH fluorescence lifetime in skeletal muscle via fiber-coupled time-correlated single photon counting[J]. Journal of Innovative Optical Health Sciences, 2024, 17(1): 2350030
Category: Research Articles
Received: Jul. 13, 2023
Accepted: Nov. 20, 2023
Published Online: Feb. 28, 2024
The Author Email: Muldoon Timothy J. (tmuldoon@uark.edu)