The degree of order and uniform of substrates is the key to surface-enhanced Raman scattering study. The structure of porous alumina template by anodic oxidation method is ordered and uniform and the template could be used as the substrate of SERS. Nanowire arrays of Ag were fabricated from the template of porous alumina. The authors observed the SERS signals of the Rh6G adsorbed on the Ag nanowire arrays. The results revealed that the SERS intensity depended on the length of the wires that emerged on the surface that was controlled by the etching time, but the frequency was not affected by the surface condition; the results also revealed that PO3－4 can increase the intensity of SERS signals.