Acta Optica Sinica, Volume. 26, Issue 10, 1543(2006)
Research on Static Fluorescence Polarization Spectrum of Whole Blood
The static fluorescence polarization spectrum of human whole blood whose concentrations are from 0.3% to 10% at the band 350~800 nm induced by 408 nm polarized light of LED is reported. The spectral characteristics and redshifted phenomenon for different concentrations of the sample solutions are discussed, and the mechanism of the red shift is also given. The polarization degrees of two key fluorescence emission bands (around 490 nm and 610 nm) in polarization spectrum are measured. The result shows that the polarization degrees of two key flourescence emission bands are 0.4796 and 0.4344 when the concentration of blood is 1.0%, and the polarization degrees are 0.2064 and 0.0538 when the concentration is 8.0%. The variation rule of the polarization degree with the concentrations of whole blood is studied. The different energy transfer mechanisms as well as the variation of polarization degree in different fluorescence areas for different concentrations of whole blood is studied by the theory of energy transfer. The research will gives reference to lightinduced autofluorescence diagnostic techniques of organic tissue.
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[in Chinese], [in Chinese], [in Chinese], [in Chinese], [in Chinese]. Research on Static Fluorescence Polarization Spectrum of Whole Blood[J]. Acta Optica Sinica, 2006, 26(10): 1543