A manufacturing-aid optical coherence tomography (M-OCT) method is proposed to achieve imaging with high-resolution, non-destructivity, full-depth, and large-volume. This method combines the discrete manufacturing principle and microtomography. High-resolution images are obtained by OCT in the discrete manufacturing process. OCT scan and discrete manufacturing are conducted simultaneously or alternately, which combines the image mosaic algorithm, the full-depth three-dimensional (3D) image of the manufacturing samples is obtained at the same time as the manufacturing is completed. Polymer scaffolds are constructed using the 3D printing technology for M-OCT verification, and the imaging results are consistent with those of microcomputed tomography (Micro-CT). Moreover, when compared with Micro-CT, M-OCT can more clearly distinguish the stack-fusion state of adjacent layers of materials and the manufacturing defects. The thickness of the printing layer, the pore diameters and the strut diameters of different regions are quantified using the high-resolution imaging results of M-OCT. The large-volume and high-resolution OCT provides a new method for monitoring the discrete manufacturing processes such as 3D printing, cell assembly, biological manufacturing, and automatic biopsy and the non-destructive tests.