In the optical characterization of micro/nano materials, in order to obtain higher resolution wavelength and intensity fluorescence images, a common imaging microscope, spectrometer and nano mobile station are combined to form a laser scanning microscope imaging system. LabVIEW is used to develope a complete set of system PC software for integrating 2D scanning acquisition and signal image processing. The scanning acquisition process uses digital signal processing methods such as low-pass filtering to eliminate the effects of spectrometer signal noise. The system is used to measure the cadmium selenide nanobelts and monolayer molybdenum disulfide. The fluorescence intensity image and the fluorescence peak wavelength image are obtained. Fluorescence with a minimum wavelength of 0.03 nm can be resolved. The acquisition length is compared with the actual length and the fluorescence intensity difference is analyzed. Good results are obtained.