Spectroscopy and Spectral Analysis, Volume. 29, Issue 2, 399(2009)
SERS Spectra of Serum from Diabetic
The SERS spectra were measured from normal and the diabetic serum.In the diabetic serum,the band of amide II shifted to 1 585 cm-1 and the relative intensity increased 14%,while the relative intensity of 593 cm-1 which belongs to amide Ⅵ reduced 33%.For the protein side chain,the band at 1 368 cm-1 assigned to the “buried” tryptophan shifted to 1 365 cm-1 of the “exposed” and the relative intensity reduced 59%. The relative intensity of 635 cm-1 assigned to the gauche conformation of the C—S decrased 15% and the band at 725 cm-1 increased 58%.These indicate that the structure of the protein changed in the diabetic serum.The relative intensity at 1 449 cm-1 assigned to the lipids characteristic increased 58%.The relative intensity of the glucide characteristic at 1 331,1 099 and 740 cm-1 increased 35%,100% and 62%,respectively.So it is indicated that the content of lipids,glucide and protein increased in diabetic.These results may offer a powerful experimental basis for diabetes diagnosis and biochemistry mechanism study.
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HAN Hong-wen, YAN Xun-ling, LI Shu-feng, BAN Ge, LI Ke, ZHANG Xia, YANG Ying, BING Yan-xia. SERS Spectra of Serum from Diabetic[J]. Spectroscopy and Spectral Analysis, 2009, 29(2): 399
Received: Sep. 6, 2007
Accepted: --
Published Online: Dec. 9, 2009
The Author Email: Hong-wen HAN (hongwen411@163.com)