In order to solve the problem of long-time operation of a fiber-based frequency comb, the control method of locking the repetition rate (f_r) and carrier envelope phase offset frequency (f₀) of optical frequency comb is improved. Two thermo-electric coolers (TEC), an upper one and an lower one, are used to control the temperature of frequency comb. By adjusting the TEC temperature, the stability of f_r and f₀ can be confined in the range of 10 and 600 Hz, respectively. Then, f_r and f₀ were further stabilized by the feed-back control of the optical path and the pump power, respectively. Finally, in 170-hour operation, the standard deviations of f_r and f₀ were measured as 0.83 and 280 mHz, respectively. The results show that the method can realize the long-term operation of the optical frequency comb and enhance its adaptability to the environment.