Surface-enhanced Raman spectroscopy (SERS) technique was used to analyze the Raman peaks of thiabendazole pesticides in the present paper. Surface enhanced substrates of silver nanoparticle were made based on microwave technology. Raman signals of thiabendazole were collected by laser Micro-Raman spectrometer with 514.5 and 785 nm excitation wavelengths, respectively. The Raman peaks at different excitation wavelengths were analyzed and compared. The Raman peaks 782 and 1 012 at 785 nm excitation wavelength were stronger, which were C—H out-of-plane vibrations. While 1 284, 1 450 and 1 592 cm-1 at 514.5 nm excitation wavelength were stronger, which were νring and CN stretching. The study results showed that the intensity of Raman peak and Raman shift at different excitation wavelengths were different. And strong Raman signals were observed at 782, 1 012, 1 284, 1 450 and 1 592 cm-1 at 514.5 and 785 nm excitation wavelengths. These characteristic vibrational modes are characteristic Raman peaks of carbendazim pesticide. The results can provide basis for the rapid screening of pesticide residue in agricultural products and food based on Raman spectrum.