The Yb3+ doped microstructure fiber is prepared by the method of high temperature plasma non-chemical vapor deposition combined with the solution doping method. Its loss is 7.5 dB/m at the wavelength of 976 nm which indicates it has good absorption efficiency at this wavelength. In order to test its laser performance, fluorescence properties are measured and a back-end pump laser amplifier system is constructed. Using an optimized 3 m length of the fiber and the seed femtosecond laser with 150 fs pulse width, 50 MHz repetition frequency and 1030 nm central wavelength, the femtosecond laser of 138 mW is successfully amplified to 605 mW, and the mode is in conformity with the Gaussian distribution. The results verify the feasibility of the doped fiber preparation method, make contributions to the exploration of new preparation methods of doped microstructure fiber and lay the foundations for high power Yb3+ doped femtosecond laser amplifier and high power lasers.