Atmospheric correction of remote sensing image is the premise of quantitative remote sensing. The present paper evaluated the capability of FLAASH (fast line-of-sight atmospheric analysis of spectral hypercubes)in ENVI software to make atmospheric correction for EO-1 Hyperion hyperspectral image. Hyperion hyperspecreal image of Zhangye city in Heihe River valley of Gansu province, China was acquired on September 10, 2007. Canopy spectra, biochemical component and GPS data of 41 plots were measured in near real-time during the satellite overpass. Hyperion hyperspectral image was geometrically corrected using Lansat-7 ETM+ image, then DN values were transformed to radiance and apparent reflectance, and atmospheric correction of Hyperion image was made using FLAASH. The resulting radiance, apparent reflectance and reflectance after FLAASH of four typical objects, including corn, water body, desert and building, were compared. ASD spectra of corn were resampled to Hyperion corresponding bands using Gaussian filter function. The comparison between ASD resampled spectra and Hyperion spectra after FLAASH demonstrated that the atmospheric correction using FLAASH is very effective and these two spectra are consistent with each other and the correlation coefficient reached 0.987.