Cytogenetic experiment is a required course for plant production majors. The traditional teaching method of it is generally based on the verification experiment, which is difficult to stimulate students’ practical motivation and recognition of the major. Considering the disadvantages of confirmatory experiment, the confirmatory experiments are designed as exploratory experiments, and both in and out of the classroom with online teaching are conbined. In this class, students are considered as “scientists”, and are encouraged to participate in the whole experimental process. This type of teaching pays attention to students’ personality development and experience and feeling, and let students become the leader of the experiment, which stimulate students’ learning motivation and professional interest, effectively enhance their innovative ability.