In order to obtain cells image, we use Visual Studio C# to develop the control program of the mobile displacement platform, and use displacement single-point scanning way to design laser confocal scanning microscope (LCSM). In order to obtain high resolution displacement, displacement of the platform with piezoelectric ceramic actuator is used and the highest accuracy can reach 1 nm. Both comb and rectangle scanning paths are designed, by programming the displacement compensation method to make up the mechanical movement deviation. Digital filtering arithmetic average method of dealing with the data acquisition card is used to collect data to reduce the influence of random noise. Experimental results show that single-point platform using C# program LCSM has a very good effect.