A two-stage registration algorithm combining global registration and local registration technology is proposed. In this algorithm, bifurcation structures with 4 connected bifurcation points rather than single bifurcation points are used as the registration features. By reducing pairing points, the efficient of registration is improved. Moreover due to non-linear deformation, there are some registration errors appear in some local region. To solve this problem, a local registration technology is introduced according to the global registration results, which improve registration precision. Experiment results show that the algorithm effectively achieve fundus image registration with higher efficient and precision.