Acta Photonica Sinica, Volume. 46, Issue 9, 923003(2017)
Study of the Fluorescent Aptasensor Based on Fluorescence Resonance Energy Transfer
One fluorescent aptasensor based on the fluorescence resonance energy transfer was constructed and used to detect insulin and dopamine. Aptamer labeled FAM (aptamer-FAM) was in charge of energy donor, while graphene oxide served as an energy acceptor. The results show that the linear detection range of insulin is from 0.05 μmol/L to 10 μmol/L and dopamine is from 1 μmol/L to 500 μmol/L, meanwhile the signal intensity of insulin is stronger than dopamine at the same concentration. The specificity experiments for insulin and dopamine have indicated that this fluorescent aptasensor have a good specificity. Due to its detection for different targets and high selectivity, this fluorescent aptasensor offers a promising prospect in the detection field of biological and medical.
Get Citation
Copy Citation Text
JIANG Li-ying, LIU Shuai, REN Lin-jiao, ZHANG Pei, YAN Yan-xia, ZHENG Xiao-wan, CHEN Qing-hua, JIANG Su-xia. Study of the Fluorescent Aptasensor Based on Fluorescence Resonance Energy Transfer[J]. Acta Photonica Sinica, 2017, 46(9): 923003
Received: Dec. 28, 2016
Accepted: --
Published Online: Oct. 16, 2017
The Author Email: Li-ying JIANG (jiangliying@zzuli.edu.cn)