A camera calibration method based on the flexible spliced two-dimensional target is proposed to orercome the problems in the camera calibration that a large scale target with high precision is hard to fabricate, and the smaller one cannot satisfy the required accuracy. According to this problem. The flexible spliced two-dimensional target consists of many small planar targets (called sub-target). Each sub-target includes a matching area and an extending area. Those sub-targets are combined into a virtual large scale planar target (called mother-target) by encode in the matching area and the camera parameters are calibrated by the mother-target. Simulation and real experiment results indicate that the accuracy of the proposed calibration method is almost the same as that of Zhang’s calibration method using a large planar target with the point numbers similar to that of the flexible spliced two-dimensional target. The relative error of the calibration result of them is less than 2%. This method shows his advantages in that the target is convenient to be fabricated, and the area of target can be extended infinitely.