Surface plasmon resonance (SPR) is a phenomenon which occurs when light is reflected off thin metal films, and the intensity of reflected light is correlated with the optical wavelength of incidence, angle of incidence, and refractive index of the material on the surface of Au film. Biochemical analysis instruments based on surface plasmon resonance have been developed for characterizing and quantifying biomolecular interaction. The working principle, technical parameters and testing method of the HPSPR-6000 were discussed here. The SPR response of immobilizating sulfamethazine antign on the surface of chip was tested and the binding value between antign and antibody on the surface chip was measured.