This study focused on the UV-Visible absorption spectra and the Fourier-infrared spectroscopy of hemoglobin (Hb) in healthy humans and analyzed its spectral characteristics to obtain the UV-Visible absorption spectrum and FTIR of solid Hb with a stable structure in healthy humans. The 21～80-year-old subjects were divided into 4 groupstocollect blood samples. Hemoglobin was purified by G-75 glucan after centrifugation, Structure-stable Hb solids were obtained by the freeze-drying method, and Hb of four groups were determined Ultraviolet-visible absorption spectroscopy and FTIR spectroscopy. QC comparative analysis, full-spectral characterization and secondary structure analysiswere used to obtain the UV and FTIR spectra of healthy human Hb. The analysis showed that the Hb UV-visible absorption spectra and FTIR spectra were the same between the four groups. The matching between the QC and the FTIR spectra was as high as 95%. The UV spectra of all the samples had five characteristic absorption peaks, and the absorption peaks absorbance and wavelength of the position were same. The FTIR spectrum had 13 characteristic absorption peaks, and the absorbance and wave number of the position of the absorption peak between different samples were same; preliminary analysis of Hb ultraviolet absorption spectrum showed that healthy human Hb had the same tertiary structure. The characteristic absorption band of FTIR spectrum Amide Ⅰ band had second derivative spectrum matching degree of up to 99%, and its secondary structure was composed of 11 sub-peaks, and the composition was mainly α-helix. The UV-Vis absorption spectroscopy and FTIR spectroscopy of the structurally stable solid human Hb were obtained. The spectroscopy and structural characteristics of Hb were obtained through preliminary analysis, which was helpful to study related diseases.