Spectral calibration is a key to realize the quantification of hyperspectral remote sensing. Traditionally, the spectral calibration for a hyperspectral sensor is performed by a monochromator scanning method with disadvantages of expensive, time-consuming,etc.,which is difficult to achieve the real-time and high-accuracy spectral calibration in the field or in-flight.In this paper,an accuracy and fast system level spectral calibration method was described for determining the center wavelengths for every spectral channel simultaneously. The reflective signals of a diffuse reflectance standard panel and a diffuse reflectance standard panel doped with a rare earth oxide were measured by the hyperspectral sensor. The ratio of two signals was optimized to remove the effects of sensor spectral response and lamp spectral output. By assuming a certain parametric model of the sensor and performing a nonlinear least squares fit of the measured ratio to the expected ratio, the center wavelength and bandwidth could be derived. A field spectroradiometer, FieldSpec UV/VNIR,was calibrated by proposed method,and the factors that effect on the calibration accuracy were analyzed. The results show that the wavelength accuracy is less than 0.5 nm,which indicates that the technology can meet the urgent needs of high accuracy and fast spectral calibration of hyperspectral sensors.