A novel agrose/silica composite was prepared via hydroxylation and poly-condensation reaction in sol-gel process with oxide agrose and tetramethoxysilane as precursor. The agrose/silica composite were functioned with amide through ring opening, “thiol-ene” click and dehydration reactions. The constitution, structure and morphology of the composite were characterized by FT-IR, XPS and SEM. Amide group modified agrose/silica composite was applied as sorbent for ractopamine and several factors such as sample solvent, adsorption time and initial concentrations of ractopamine were investigated. The results show that amide group was successfully modified onto the composite. Meanwhile, continuous porous structure was formed and the particle size was about 2-3 μm. The composite show excellent adsorption property for ractopamine. The equilibrium could be reached in 50 min and adsorption behavior belonged to the pseudo-second- order kinetic model. Chemical adsorption was assumed. The adsorption isothermal is in accordance with Freundlich model. The adsorption property decreases a little affter the 6 cycle adsorption experiments.