The high incidence and mortality of pancreatic adenocarcinoma have attracted widespread attention in recent years. However, the existing detection methods are costly and complex to operate, which makes it difficult to extend their use to households in the community. On the other hand, the lower-cost traditional immunochromatographic test strips are difficult to detect the markers in blood samples effectively. A microfluidic-immunochromatographic test strip for tumor marker CA199 was designed. With the aid of the optical microscope, the microarray structure could effectively separate red blood cells in sample solution without destroying the cellular structure. The physical properties of the quantum dot fluorescent microspheres were analyzed by a transmission electron microscope and a multifunctional enzyme marker. The study shows that the quantum dot fluorescent microspheres have good stability and dispersity. By comparison with unfiltered samples, the enhancement of microfluidic structures for CA199 fluorescent signals in samples was demonstrated. The detection limit of the system is 4.2 U/mL. The microfluidic-immunochromatographic test strips are low-cost, simple-to-operate, safe and stable. It provides a new strategy for home-based screening for pancreatic adenocarcinoma.