To solve the problem of large geo-location error in the application of ground target geo-location in long-distance aviation imaging with large inclination angle, a systematic error corrected geo-location was proposed. The systematic error analysis and modeling of the long-distance aviation imaging system with large inclination angle was carried out. In view of the difficulty in obtaining the residual error, a method for estimating residual error parameters based on ground control points was proposed.The simulation results show that the residual error can be reduced to 1/10 by estimating residual error parameters based on ground control points. Flight test data confirm that the mean geo-location error can be reduced from 401 m to 97 m by correcting the systematic error in the positioning of targets in long-distance aviation imaging with large inclination angle. Thus, the systematic error corrected geo-location method can effectively improve the accuracy of ground target geo-location in the application of long-distance aviation imaging with large inclination angle.