In recent years, with the gradual deepening of researches on the field of micro-nano integration such as microfluidics, micromachines, and microelectronics, joining among different kinds of materials has become a bottleneck in the related fields. Due to the characteristics of controllable thermal effect and high precision, ultrashort pulsed laser welding has become a hotspot in the welding of non-metallic materials. In this review article, applications and concrete cases of the welding of non-metallic materials such as glasses, ceramics, semiconductor monocrystalline materials, and organic polymers are introduced. In addition, the future trends of ultrashort pulsed laser welding are also prospected.