The relationship of scattering coefficient and concentration of blood glucose is discussed. The scattering coefficient of turbid media is determined by the relative refractive index of scattering particles to solvent. The variation of blood glucose concentration changes the refractive index of blood, and the scattering coefficient of the blood. It is given that the formuta to calculate the variation of scattering coefficient induced by concentration change of blood glucose concentration based on theoretical analysis, which shows that the scattering coefficient is directhy proportional to the blood gloucose concentration. The measurement accuracy of scattering coefficient relative change should attain 4.8×10-4 to satisfy the requirement of clinical use, and for Chinese, the absolute measurement precision must be higher than 6.82×10-3 mm-1. Double integrating sphere experiment is used to validate the theory. Milk is used as turbid media to observe the relationship of scattering coefficient and glucose concentration. At every wavelength the scattering coefficients are linearly changed with the glucose concentration and the linearity value is higher than 0.95. The theoretical analysis and the experimental result prove that blood glucose concentration can be measured by scattering coefficient.