Acta Photonica Sinica, Volume. 40, Issue 5, 722(2011)
Sorting Economic Microorganisms by Using Optical Manipulation and Raman Microspectroscopy
A novel techique of sorting yeast cell based on Raman characteritics of target products synthesized by microorganisms was developed by using Raman microspectroscopy coupled with optical manipulatation. To sort oleaginous yeast and carotenoid-producing yeast, the plant oil and β-carotene were chosen as the target products and their Raman characteritics, the intensity ratios of 1 440 cm-1 and 1 602 cm-1 band (i.e. I1440/I1602) for oil, and I1157/I1654 or I1520/I1654 for carotenoid, were extracted and marked as target cells. Single yeast cell was captured and manipulated by using a 780 nm laser, and the Raman spectra of trapping cell was acquired simultaneously.A real-time computer distinguishing program was used to assist the sorting. The distinguished cells were then extracted and validated by following culture and Raman detection. This work provides a new method for screening microorganisms synthesize products with economic value.
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LI Zi-da, TAO Zhan-hua, MENG Ling-jing, CHEN Shan-shan, LIU Jun-xian, WANG Gui-wen. Sorting Economic Microorganisms by Using Optical Manipulation and Raman Microspectroscopy[J]. Acta Photonica Sinica, 2011, 40(5): 722
Received: Nov. 24, 2010
Accepted: --
Published Online: Jun. 14, 2011
The Author Email: Zi-da LI (lizida23@163.com)