The echo amplitude of near-field separated multiple-input multiple-output （ MIMO） radar is different due to the different angle of each channel relative to the target. Therefore, a method of echo amplitude calibration based on beam pointing adjustment is proposed. By using the conversion relationship between the antenna beam pointing and the echo amplitude change, the indirect adjustment of the antenna echo amplitude is realized by adjusting the beam pointing so that the difference of echo amplitude between channels could be calibrated. The proposed method is verified by using a conventional geometrically separated MIMO array. The results show that the imaging performance of near-field separated MIMO radar can be improved significantly after the calibration of echo amplitude difference.