Aiming at the unknown fatigue performance of fiber Bragg grating(FBG) strain sensors used in crane structure health monitoring, the fatigue performance of three packaged FBG strain sensors used in crane structure fatigue damage monitoring is studied by combining dynamic load with static tensile test method. The sensitivity coefficient, bandwidth, edge rejection ratio, peak-valley ratio, zero-point wavelength and dynamic strain of the sensor are analyzed with the increase of fatigue frequency. The test results show that before and after fatigue, when loading and unloading the same load, the difference of sensitivity coefficients is no more than 0.1 pm/με, the mean bandwidth is 0.19 nm to 0.23 nm, the peak to trough ratio and edge rejection ratio change with the increase of fatigue frequency, and the maximum change of zero wavelength is 0.31 nm. The dynamic strain conforms to the distribution characteristics of quadratic exponential function.