Five different pretreatment methods, including dry ashing and microwave digestion with four acid systems (HNO3+H2O2, HNO3+H2O2+HF, HNO3+HClO4 and HNO3+HClO4+HF), were employed for digestion of the samples of Rosa rugosa. Nine major and trace elements were determined using ICP-OES method. The addition standard recovery rates indicate that, with the system of HNO3+HClO4, better results can be obtained for most of the determined elements. With this method, except for Fe, the recovery rates are in the range of 95.4%～104.6%. For Fe, higher recovery rate (99%) was obtained with HNO3+H2O2+HF system.