Due to its unique advantages of rapid, high efficiency, non-contact detection and no need for sample pretreatment, Raman spectroscopy is an ideal method for high-throughput, non-destructive on-line detection of large quantities of samples and a suitable method for rapid detection of ethanol concentration of liquor products. However, different from pure water, besides Raman characteristic peaks of ethanol and water, there is apparent fluorescence interference within the spectra of most liquor under laser excitation, which negatively influences the quantitative determination of ethanol concentration. Therefore, during the determination of ethanol concentration based on the Raman characteristic peak intensity ratio method, the data points for fluorescence background fitting need to be manually selected before data processing, which possess strong subjectivity and low data processing efficiency and is difficult to fully meet the technical requirements of the high-throughput samples on-line screening. In order to solve the above problems, based on the self-built laser-induced polarized Raman spectroscopy detection system, a series of experiments were carried out on the polarization characteristics of the Raman peaks and fluorescence background of four kinds of liquor samples under excitation of linearly polarized laser with different polarization orientation. Based on the polarization difference of them, a quantitative determination method of alcohol concentration in liquor products based on polarized Raman spectroscopy is proposed. The experimental results show that, with the aid of differential polarization Raman spectroscopy, the correlation coefficient of the polynomial fitting of three times is over 0.99, which can achieve accurate inversion of ethanol concentration in the range of 3%~97%vol. The accuracy of ethanol concentration in four kinds of liquor samples is significantly higher than the inversion results of traditional methods, which significantly improves the accuracy and efficiency of alcohol concentration determination of liquor products.