Laser & Optoelectronics Progress, Volume. 58, Issue 5, 0526001(2021)
Portable Fluorescence Detection System for Measuring Pepsinogen I
Pepsinogen I in human serum is a common biomarker for diagnosing gastroesophageal reflux in clinical medicine. Traditional detection methods for pepsinogen I have the disadvantages of being time-consuming and having high cost and low sensitivity. In this study, a fluorescence detection system for the rapid and quantitative measurement of pepsinogen I is designed. First, a portable microscope is used to take a fluorescence image of the samples, and then the R channel image-processing algorithm of the RGB color mode is used to detect and analyze the images. Finally, according to experimental data, a standard curve is fitted with a fitting degree of 99.556%, and the variation coefficient of the test results is <4.9%. The experimental results show that the designed detection system can accurately and rapidly quantify pepsinogen I concentration.
Get Citation
Copy Citation Text
Mingming Xu, Wei Shen, Hua Xia, Yi Wu, Hui Xue, Sujuan Chen, Xiaole Luo, Jiapeng Gu. Portable Fluorescence Detection System for Measuring Pepsinogen I[J]. Laser & Optoelectronics Progress, 2021, 58(5): 0526001
Category: Physical Optics
Received: Jun. 3, 2020
Accepted: Aug. 3, 2020
Published Online: Apr. 19, 2021
The Author Email: Xia Hua (huaxia@aiofm.ac.cn)