Laser & Optoelectronics Progress, Volume. 54, Issue 8, 80402(2017)
Microlens Array Applied for Laser Induced Fluorescence Detection
Microfluidic systems based on laser induced fluorescence (LIF) detection technology have been widely used in the field of biochemistry detection. Aiming at the problem of weak fluorescence intensity induced by a few detection samples in the microfluidic system, a microfluidic chip integrating a microlens array (MLA) is designed and fabricated to improve the intensity of the detected fluorescence. An 8×8 photoresist MLA mold with the diameter variation coefficient of 0.36% is obtained by the hot melt technology. Then, a cover plate integrating a polydimethylsiloxane MLA is produced by the soft-lithography technology, and the focal length uniformity error is 7%. Afterwards, a micro-channel substrate is made. The substrate and the cover plate are packaged by the oxygen plasma bonding technology. Finally, the fluorescence intensity of the chip is detected by a fluorescence microscope, and the chip is injected by fluorescein isothiocyanate fluorescent dye solution with concentration of 10 μmol·L-1. The result shows that the fluorescence intensity with the microlens is improved about 2.2 times as much as the fluorescence intensity without the microlens.
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Zhang Xuehai, Liu Chong, Liang Chao, Meng Fanjian, Li Jingmin. Microlens Array Applied for Laser Induced Fluorescence Detection[J]. Laser & Optoelectronics Progress, 2017, 54(8): 80402
Category: Detectors
Received: Feb. 6, 2017
Accepted: --
Published Online: Aug. 2, 2017
The Author Email: Xuehai Zhang (zhangxh@mail.dlut.edu.cn)