Cs4SrI6∶3% Eu scintillation crystal was successfully grown by vertical Bridgman method. The rough crystal size reaches up to 25×70 mm3, it is the largest Cs4SrI6∶Eu single crystal to date. XRD results show that the crystal has a K4-CdCl6 crystal structure and belongs to the R-3C space group.The fluorescence properties of the crystal were studied by the ultraviolet-visible fluorescence spectrum and X-ray excitation emission spectrum. The scintillation performance and decay time of Cs4SrI6∶Eu single crystal under 137Cs 662 keV radiation were studied. The results show that the crystal has a high light output and excellent energy resolution, decay time is about 1.86 μs. By analyzing the concentration of Eu2+ in different parts of the crystal, calculate the segregation coefficient of Eu2+ in Cs4SrI6∶Eu crystal is 1.136. The results show that Cs4SrI6∶Eu crystal has potential application prospects in radiation detection.