A new design approach to function parameters of pupil filter based on optimization algorithm is proposed. Out of regard for practical request for optical energy and sidelobe intensity and superresolution,the appropriate parameters are selected to achieve the best three-dimensional superresolution by means of the computer simulation. Some superresolution optimization design models and examples are presented with figures testification. To suppress the sidelobe intensity which is concomitant with the increase of the resolutions,these filters are used in confocal scanning microscopic system,and as a result,higher signal-tonoise and good image contrasts are obtained. With applying optimization algorithms,the design of pupil filters becomes simpler,and possesses better comprehensive effects. The pupil filters,which are designed with the aid of optimization algorithm,possess larger G_A,G_T,S. Theoretical and practical examples prove the method is efficient and adaptable.