One type of multi-spectrum imaging system based on film interference technique is discussed. A prototype of multi-spectrum imaging machine is developed, which consists of a band-pass filter, a CCD, logic control components and computer analysis software. Experiments such as inspection of agricultural products and physical composition are carried out. The result shows that the imaging system can realize spectral image acquisition at a high resolution and analyze the reflectance spectrum reconstruction and image classification accurately. The system is also applied in the inspection experiment of mouldy beans, electrolyte composition and density, and scribbled signatures with different pens.