Light-induced delayed luminescence (DL) of living organisms contains information on the quality of the living state of these organisms. Employing a LS-55 Luminescence Spectrometer, the changes of DL spectra of rice with Aspergillus flavus treating different time were observed. Rice with Aspergillus flavus treatment for a shorter time had a stronger intensity of DL. The polluted degree of Aspergillus showed a negative correlation with the intensity of DL. Comparing with the intensity of DL, we found that the concentration of aflatoxin in different polluted degree rice had negative correlation with the intensity of DL. We believe DL technique may be helpful in elaborating a fast, holistic, and non-invasive method for the rapid evaluation the polluted degree of rice by Aspergillus flavus.