In the present paper, the authors examined some inclusions of carbonate reservoir by microscopic confocal Raman spectral methods. The measurement temperature of sample was 18 ℃, -40 ℃, -80 ℃ and -170 ℃, respectively. The experimental results show that the original experimental spectral curve of the inclusion was very similar to the original experimental spectral curve of pure main mineral (rock core). Because the size of some inclusions was relatively small or inclusions were hidden deeply in the sample, their Raman spectra signals were often buried in other signals (noises) which stem from main mineral. To overcome the above-mentioned difficulty we used the spectral subtraction method. In Raman spectral experimental process, X-Y scanning and depth profile (Z scanning) were finished around the inclusion. The final results show that spectral subtraction method allows the study of solid samples with high precision because some corrections can be made, if additive properties of spectral quantity are satisfied. Interfering spectral signal from main mineral can be removed, and the inclusion spectrum can be purified收稿日期: , 修订日期:基金项目:作者简介: e-mail: