Chinese Journal of Lasers, Volume. 46, Issue 4, 0407001(2019)
Long-Time Stabilization Imaging by Light Sheet Fluorescent Microscope
By using a 4f (f is the focal length) optical setup and a charge coupled device camera, the sample position is tracked. The mechanical drift is compensated by the nano-translation table which ensures the sample always at the beam waist of the light sheet and thus an optimal image is obtained. With the proposed method, the mechanical drift of several nanometers can be compensated. By using the home-made light sheet fluorescence microscopy, the long-time imaging of fluorescent microspheres with diameters of several micrometers is acquired, which confirms the feasibility of the proposed method.
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Qiu Zhang, Dong Liang, Lihua Bai, Jun Liu. Long-Time Stabilization Imaging by Light Sheet Fluorescent Microscope[J]. Chinese Journal of Lasers, 2019, 46(4): 0407001
Category: biomedical photonics and laser medicine
Received: Dec. 27, 2018
Accepted: Jan. 18, 2019
Published Online: May. 9, 2019
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